mouse liver  (10X Genomics)

Journal: Journal of Advanced Research

Article Title: The GOLM1-ACLY pathway regulates macrophage-secreted EFEMP1 via H3K27ac modifications to drive tumor progression

doi: 10.1016/j.jare.2025.07.003

Figure Lengend Snippet: GOLM1 expression is upregulated in macrophages and correlates with tumor progression (A) Representative H&E and immunofluorescence microscopy images of carcinoma and para -carcinoma tissue of lung cancer patients. Red, CD68; green, GOLM1; blue, DAPI. Upper row, scale bar = 500 μm; lower row, scale bar = 50 μm. (B) Analyzing the co-localization ratio of CD68 and GOLM1 using Pearson’s correlation coefficients via ImageJ with the JAcop plugin. 10 visual fields from 5 pairs of carcinoma and para -carcinoma tissues were examined. (C) Representative immunofluorescent staining of F4/80 and GOLM1 in liver sections from AAV- Golm1 or AAV-vector infected mice. Scale bar = 100 μm. (D) GOLM1 protein levels in BMDMs derived from AAV- Golm1 or AAV-vector infected mice. (E) Schematic diagram of orthotopic HCC tumor model established by injecting Hepa1-6 cells into the liver of AAV- Golm1 or AAV-vector infected mice. (F) Body weight growth curves of mice inoculated with tumor. n = 6 mice per group. (G) Photographs of liver organs with HCC nodules from AAV- Golm1 and AAV-vector mice at terminal growth size. Scale bar = 40 mm. (H) Analysis of the ratio of tumor area to tissue area. n = 6 mice/group. (I) Representative H&E and Ki67 staining images of liver tumor in AAV- Golm1 or AAV-vector infected mice. Red, Ki67; blue, DAPI. Scale bar = 60 μm. (J) Quantitative analysis of the Ki67 positive area. n = 5 mice/group. (K and L) Immunoblotting and statistical graph of the indicated protein levels in tumors of AAV- Golm1 mice and AAV-vector mice. n = 3 mice/group. The data are presented as the means ± SEMs. Statistical significance was determined by two-tailed Student's t test in (B, H, J, and L) and two-way ANOVA followed by Bonferroni's multiple comparisons test (F). ns, not statistically significant. *p < 0.05; **p < 0.01; ***p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The human HCC cell lines Huh7 and HepG2; the human macrophage lines Thp1and HEK293T and the mouse liver cancer cell line Hepa1-6 were originally obtained from the ATCC.

Techniques: Expressing, Immunofluorescence, Microscopy, Staining, Plasmid Preparation, Infection, Derivative Assay, Western Blot, Two Tailed Test

Journal: Journal of Advanced Research

Article Title: The GOLM1-ACLY pathway regulates macrophage-secreted EFEMP1 via H3K27ac modifications to drive tumor progression

doi: 10.1016/j.jare.2025.07.003

Figure Lengend Snippet: Macrophage-specific knockout of Golm1 impedes tumor proliferation (A) Schematic representation of orthotopic HCC tumor model established by injecting Hepa1-6 cells into the liver of Golm1 WT or Golm1 MKO mice. (B) Hepa1-6 tumors in Golm1 WT and Golm1 MKO mice at terminal growth size. Scale bar = 40 mm. (C) Analysis of the ratio of tumor area to tissue area. n = 6 mice/group. (D) H&E and Ki67 immunohistochemical staining of liver tumors in Golm1 WT and Golm1 MKO mice. Main image, scale bar = 200 μm; zoomed regions, scale bar = 40 μm. (E) Ki67 positive rate in tumors evaluated by immunohistochemistry analysis. n = 6 fields. (F-G) Representative bands (F) and quantitative analysis (G) of relative protein expression in tumors of Golm1 WT and Golm1 MKO mice. n = 3 mice/group. (H) GSEA enrichment analysis of cancer related pathways based on KEGG. n = 3 mice/group. (I) Schematic representation of the Thp1 nc -Huh7 or Thp1 −/− -Huh7 admixture tumor model on nude mice. (J) Tumor growth curves of subcutaneous models involving Huh7 cells cocultured with Thp1 nc or Thp1 −/− cells. n = 5 nude mice in each group. (K and L) Tumor images (K) and weight (L) of subcutaneous tumor in groups of nude mice. Scale bar = 20 mm. n = 5 nude mice in each group. (M and N) Representative bands (M) and quantitative analysis (N) of relative protein expression in tumors of Huh-7, Thp1 nc -Huh7 or Thp1 −/− -Huh7 nude mice. n = 3 mice/group. The data are presented as the means ± SEMs. Statistical significance was determined by two-tailed Student's t test (C, E, and G) and one-way ANOVA followed by Tukey's multiple comparisons test (J, L, and N). ns, not statistically significant. *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: The human HCC cell lines Huh7 and HepG2; the human macrophage lines Thp1and HEK293T and the mouse liver cancer cell line Hepa1-6 were originally obtained from the ATCC.

Techniques: Knock-Out, Immunohistochemical staining, Staining, Immunohistochemistry, Expressing, Two Tailed Test